Deoxynivalenol downregulates NRF2-induced cytoprotective response in human hepatocellular carcinoma (HepG2) cells
نویسندگان
چکیده
Deoxynivalenol (DON) commonly infects agricultural foods; it exhibits toxicity by inducing oxidative stress and inhibiting protein synthesis. Nuclear factor erythroid 2-related 2 (NRF2) regulates the cellular antioxidant response. We investigated cytotoxicity of DON its effect on NRF2 response in HepG2 cells. The Methyl Thiazol Tetrazolium (MTT), glutathione (GSH) ATP assays evaluated toxicity, whilst lipid peroxidation membrane damage were assessed using Thiobarbituric acid reactive substance (TBARS) lactate dehydrogenase (LDH) assays. Protein expression NRF2, phosphorylated (p-ser40) catalase (CAT), superoxide dismutase (SOD2), Sirtuin 3 (Sirt3) quantified Western Blotting. Gene peroxidase (GPx), CAT SOD2 was determined qPCR. decreased cell viability, GSH concentrations levels increased damage. significantly total p-NRF2 downregulated transcription translation target enzymes. Further, mitochondrial protein, Sirt3 decreased. In conclusion, induced NRF2-induced cytoprotection suppressing signalling mechanism
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ژورنال
عنوان ژورنال: Toxicon
سال: 2021
ISSN: ['0041-0101', '1879-3150']
DOI: https://doi.org/10.1016/j.toxicon.2021.01.017